PSMD6 antibody

Catalog No. ABIN2452115

Product Details anti-PSMD6 Antibody

Target: PSMD6 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 6 (PSMD6))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD6 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Characteristics: Product: Rabbit polyclonal antibody affinity purified with recombinant Rpn7p

Purification: Affinity purified

Immunogen: Recombinant yeast Rpn7p expressed in E. coli

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: ~1000 fold dilution
2) Immunoprecipitation Not tested for other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCl, 10 mM Tris-HCl pH 7.4, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: To avoid repeated freezing and thawing, store the antibody solution in aliquots.

Storage: -20 °C

Storage Comment: -20 C. To avoid repeated freezing and thawing, store the antibody solution in aliquots.

References for anti-PSMD6 antibody (ABIN2452115)

Isono, Nishihara, Saeki, Yashiroda, Kamata, Ge, Ueda, Kikuchi, Tanaka, Nakano, Toh-e: "The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome." in: Molecular biology of the cell, Vol. 18, Issue 2, pp. 569-80, (2007) (PubMed).

Isono, Saeki, Yokosawa, Toh-e: "Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 279, Issue 26, pp. 27168-76, (2004) (PubMed).

Finley, Tanaka, Mann, Feldmann, Hochstrasser, Vierstra, Johnston, Hampton, Haber, Mccusker, Silver, Frontali, Thorsness, Varshavsky, Byers, Madura, Reed, Wolf, Jentsch, Sommer, Baumeister, Goldberg et al.: "Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. ..." in: Trends in biochemical sciences, Vol. 23, Issue 7, pp. 244-5, (1998) (PubMed).

Hershko, Ciechanover: "The ubiquitin system." in: Annual review of biochemistry, Vol. 67, pp. 425-79, (1998) (PubMed).

Target Details for PSMD6

Target: PSMD6 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 6 (PSMD6))

Alternative Name: Rpn7 (PSMD6 Products)

Background: Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa and is highly conserved among eukaryotic organisms. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation. Rpn7 is one of the lid subunits of the 26 S proteasome regulatory particle. The RPN7 gene is known to be essential and required for the integrity of the 26 S complex by establishing a correct lid structure.

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway