PSMD8 antibody

Catalog No. ABIN2452112

Product Details anti-PSMD8 Antibody

Target: PSMD8 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Purification: Affinity purified

Immunogen: Recombinant yeast Rpn12 expressed in E. coli

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: 1/5,000~1/10,000
2) Immunoprecipitation
Other applications have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 1 mg/mL BSA, 0.09 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-PSMD8 antibody (ABIN2452112)

Tone, Tanahashi, Tanaka, Fujimuro, Yokosawa, Toh-e: "Nob1p, a new essential protein, associates with the 26S proteasome of growing saccharomyces cerevisiae cells." in: Gene, Vol. 243, Issue 1-2, pp. 37-45, (2000) (PubMed).

Takeuchi, Toh-e et al.: "Genetic evidence for interaction between components of the yeast 26S proteasome: combination of a mutation in RPN12 (a lid component gene) with mutations in RPT1 (an ATPase gene) causes synthetic ..." in: Molecular & general genetics : MGG, Vol. 262, Issue 1, pp. 145-53, (1999) (PubMed).

Hershko, Ciechanover: "The ubiquitin system." in: Annual review of biochemistry, Vol. 67, pp. 425-79, (1998) (PubMed).

Target Details for PSMD8

Target: PSMD8 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8))

Alternative Name: Rpn12 (PSMD8 Products)

Background: Background: The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation. The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn12 is one of the non-ATPase subunits of the lid. Rpn12 interacts with an ATPase subunit, Rpt1, of the base. Rpn12, Rpt1 double mutant becomes lethal, suggesting a strong interaction between Rpn12 and Rpt1. In the double mutant cells, the function of the 26S proteasome is completely eliminated.

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Proton Transport, Synthesis of DNA, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway