Rad6 (full length) antibody

Catalog No. ABIN2452101

Product Details

Target: Rad6

Binding Specificity: full length

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity (Details): Not tested in other species.

Purification: Affinity purified with the immunogen conjugated to agarose

Sterility: Sterile filtered

Immunogen: Human Rad6B full-length recombinant protein

Application Details

Application Notes: 1) Western blotting: 1,000~2,000 fold dilution
2) Immunoprecipitation (assay dependent)
3) Immunofluoscent staining (assay dependent)
Other applications have not been tested

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References

Masuyama, Tateishi, Yomogida, Nishimune, Suzuki, Sakuraba, Inoue, Ogawa, Yamaizumi: "Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 10, Issue 8, pp. 753-62, (2005) (PubMed).

Target Details

Target: Rad6

Background: Resume of DNA synthesis, which was halted by various physical and chemical factors, was triggered by repair system including Rad6. Rad6 (152a.a, 17.3 kDa) is the ubiqutin-conjugated enzyme E2A, and complex with two Rad18 Molecules, ubiquitin ligase E3, to ubiquitinate PCNA by utilizing poly-ubiquitin chain that HLTF-UBC13 synthesized and they are involved in translesion DNA synthesis (TLS) and template switch (TS). Two isoforms of Rad6, Rad6A and Rad6B differs in only 6 amino acids. They also play an important role in epigenetic transcriptional regulation by catalyzing the monoubiquitination of histone H2B at Lys-120.

UniProt: P49459, P63146