NUP62 antibody (Phenylalanine-Glycine Repeat)

Catalog No. ABIN2452063

Product Details anti-NUP62 Antibody

Target: NUP62 (Nucleoporin 62kDa (NUP62))

Binding Specificity: AA 1-179, Phenylalanine-Glycine Repeat

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This NUP62 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Specific to human (HeLa cells) and simian (Cos cells). The antibody did not react with mouse.

Purification: Purified from serum-free culture medium of the hybridoma by proprietary chromatography under mild conditions.

Sterility: Sterile filtered

Immunogen: Recombinant human Nup62 (amino acids 1-300) (GST-Nup62-His)

Clone: 2A

Isotype: IgG1 kappa

Application Details

Application Notes: 1. Western blotting: 1/500 ~1/2,000 dilution
2. Immunoprecipitation (assay dependent)
3. Immunofluorescece / Immunocytochemistry: 1/400)
4. ELISA (assay dependent)
5. When this antibody was micro-injected into the cytoplasm of the HeLa cells, it accumulates into the nuclear pores as examined by immunofluorescence staining. .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-NUP62 antibody (ABIN2452063)

Fukuhara, Sakaguchi, Katahira, Yoneda, Ogino, Tachibana: "Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies." in: Hybridoma (2005), Vol. 25, Issue 2, pp. 51-9, (2006) (PubMed).

Maeshima, Yahata, Sasaki, Nakatomi, Tachibana, Hashikawa, Imamoto, Imamoto: "Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins." in: Journal of cell science, Vol. 119, Issue Pt 21, pp. 4442-51, (2006) (PubMed).

Target Details for NUP62

Target: NUP62 (Nucleoporin 62kDa (NUP62))

Alternative Name: Nup62 (NUP62 Products)

Background: The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nucleoporins are the main components of the NPC in eukaryotic cells. Nup (Nucleoporin) 62 (522 aa, 53 kDa) is a member of the FG-repeat containing nucleoporins and is localized to the NPC central plug. Nup62 associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Predicted to contain about 10 N-acetylglucosamine side chain .

UniProt: P37198

Pathways: EGFR Signaling Pathway, SARS-CoV-2 Protein Interactome