NUP153 antibody (AA 610-1191)
(2 references)-
- Target See all NUP153 Antibodies
- NUP153 (Nucleoporin 153kDa (NUP153))
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Binding Specificity
- AA 610-1191
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Reactivity
- Rat
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This NUP153 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Specific to human, mouse, rat and monkey Nup153 proteins.
- Cross-Reactivity (Details)
- Not tested for other species
- Characteristics
- When injected into the nucleus,R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.
- Purification
- Purified
- Sterility
- Sterile filtered
- Immunogen
- Recombinant GST-fused rat Nup153 (610-1191aa, Zn finger and FG repeats domain)
- Clone
- R4C8
- Isotype
- IgG2a kappa
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anti-Nucleoporin 153kDa (NUP153) antibody
NUP153 Reactivity: Human WB, IHC, IF Host: Rabbit Monoclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 1456-1475) antibodyNUP153 Reactivity: Human, Rat, Xenopus laevis WB, IP, IF, ICC Host: Sheep Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) antibodyNUP153 Reactivity: Human ELISA, IHC, IF Host: Rabbit Monoclonal 8D4 unconjugated Recombinant Antibody
anti-Nucleoporin 153kDa (NUP153) (AA 58-86), (N-Term) antibodyNUP153 Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB17477 unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 1238-1468) antibodyNUP153 Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 657-880) antibodyNUP153 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (C-Term) antibodyNUP153 Reactivity: Human, Rat, Mouse WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 1127-1176) antibodyNUP153 Reactivity: Human, Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 65-94), (N-Term) antibodyNUP153 Reactivity: Human WB, FACS, EIA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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1. Western blotting: 160 kDa single band in Hela cell extract
2. Immunocytochemistry
3. ELISA
Other applications have not been tested.
Additional comments:
When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.
R4C8 works in immuno-cytochemistry very well. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol
- Preservative
- Azide free
- Storage
- -20 °C
- Storage Comment
- Upon receipt aliquot and store at -20 C
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: "Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).
: "The nucleoporin nup153 plays a critical role in multiple types of nuclear export." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 649-64, (1999) (PubMed).
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: "Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).
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- Target
- NUP153 (Nucleoporin 153kDa (NUP153))
- Alternative Name
- Nup153 (NUP153 Products)
- Background
- The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153 kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.
- UniProt
- P49790
- Pathways
- Protein targeting to Nucleus
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