NUP153 antibody (AA 610-1191)

Catalog No. ABIN2452062

Product Details anti-NUP153 Antibody

Target: NUP153 (Nucleoporin 153kDa (NUP153))

Binding Specificity: AA 610-1191

Reactivity: Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This NUP153 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Specific to human, mouse, rat and monkey Nup153 proteins.

Cross-Reactivity (Details): Not tested for other species

Characteristics: When injected into the nucleus,R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant GST-fused rat Nup153 (610-1191aa, Zn finger and FG repeats domain)

Clone: R4C8

Isotype: IgG2a kappa

Application Details

Application Notes: 1. Western blotting: 160 kDa single band in Hela cell extract
2. Immunocytochemistry
3. ELISA
Other applications have not been tested.

Additional comments:
When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.
R4C8 works in immuno-cytochemistry very well.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon receipt aliquot and store at -20 C

References for anti-NUP153 antibody (ABIN2452062)

Lim, Fahrenkrog, Köser, Schwarz-Herion, Deng, Aebi: "Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).

Ullman, Shah, Powers, Forbes: "The nucleoporin nup153 plays a critical role in multiple types of nuclear export." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 649-64, (1999) (PubMed).

Target Details for NUP153

Target: NUP153 (Nucleoporin 153kDa (NUP153))

Alternative Name: Nup153 (NUP153 Products)

Synonyms: CG4453 antibody, Dmel\\CG4453 antibody, NUP153 antibody, Nup 153 antibody, dNup153 antibody, dmNup153 antibody, nup153 antibody, NUCZINK antibody, B130015D15Rik antibody, C88147 antibody, HNUP153 antibody, N153 antibody, Nucleoporin 153kD antibody, nucleoporin 153 antibody, nucleoporin 153kDa L homeolog antibody, Nup153 antibody, NUP153 antibody, nup153.L antibody

Background: The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153 kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.

UniProt: P49790

Pathways: Protein targeting to Nucleus