NOB1 antibody

Catalog No. ABIN2452059

Product Details anti-NOB1 Antibody

Target: NOB1 (RNA-Binding Protein NOB1 (NOB1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Characteristics: Product: Rabbit polyclonal antibody affinity purified with recombinant Nob1p

Purification: Affinity purified

Immunogen: Recombinant yeast Nob1p expressed in E. coli

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: ~400 fold dilution
2) Immunoprecipitation Not tested for other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 1 mg/mL BSA as carrier, 0.09 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-NOB1 antibody (ABIN2452059)

Tone, Toh-E: "Nob1p is required for biogenesis of the 26S proteasome and degraded upon its maturation in Saccharomyces cerevisiae." in: Genes & development, Vol. 16, Issue 24, pp. 3142-57, (2002) (PubMed).

Tone, Tanahashi, Tanaka, Fujimuro, Yokosawa, Toh-e: "Nob1p, a new essential protein, associates with the 26S proteasome of growing saccharomyces cerevisiae cells." in: Gene, Vol. 243, Issue 1-2, pp. 37-45, (2000) (PubMed).

Target Details for NOB1

Target: NOB1 (RNA-Binding Protein NOB1 (NOB1))

Alternative Name: Nob1 (NOB1 Products)

Background: Background: The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation. Nob1p is essential nuclear protein required for biogenesis of the 26S proteasome. Nob1p is speculated to serve as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and to be degraded upon the maturation of the 26S proteasome. Nob1p is composed of 459 amino acid residues.

Pathways: Protein targeting to Nucleus