HIV-1 p24 antibody (full length)

Catalog No. ABIN2452021

Product Details anti-HIV-1 p24 Antibody

Target: HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

Binding Specificity: full length

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This HIV-1 p24 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Characteristics: Antiserum

Purification: Guinea Pig serum

Immunogen: Purified full-size recombinant Gag p24 of HIV-1 subtype B expressed in E. coli

Application Details

Application Notes: 1. Western blot: 1/2,000~1/5000
2. Dot blot (assay depemdemt
3. Immunoprecipitation (assay dependent)
4. ELISA (assay dependent)
Other applications have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Upon receipt aliquot and store at -20 C

References for anti-HIV-1 p24 antibody (ABIN2452021)

Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).

Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

Target Details for HIV-1 p24

Target: HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

Alternative Name: HIV-1 Gag p24 (HIV-1 p24 Products)

Target Type: Viral Protein

Background: HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction. As this protein is detectable from the early stage of AIDS virus infection, it is used as a marker for observation of the patient's condition after treatment, as it indicates the amount of virus in the blood. Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells.