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HBEGF antibody (EGF Like Domain) (Biotin)

HBEGF Reactivity: Human WB, ELISA, IP, ICC, IF Host: Mouse Monoclonal 4G10 Biotin
Catalog No. ABIN2451994
  • Target See all HBEGF Antibodies
    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
    Binding Specificity
    • 15
    • 15
    • 14
    • 8
    • 7
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    EGF Like Domain
    Reactivity
    • 74
    • 20
    • 19
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 69
    • 8
    Mouse
    Clonality
    • 67
    • 9
    • 1
    Monoclonal
    Conjugate
    • 26
    • 9
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This HBEGF antibody is conjugated to Biotin
    Application
    • 33
    • 28
    • 26
    • 15
    • 13
    • 9
    • 7
    • 6
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Cross-Reactivity (Details)
    Does not react with mouse.
    Characteristics
    biotin/IgG = 7.5
    Purification
    Purified
    Sterility
    Sterile filtered
    Immunogen
    Recombinant human HB-EGF ectodomain expressed in SF21 cell
    Clone
    4G10
    Isotype
    IgG1
    Top Product
    Discover our top product HBEGF Primary Antibody
  • Application Notes
    1) Western blotting: 0. 2~1 g/mL
    2) Immunoprecipitation: 2 g/mL
    3) Indirect immuno-fluorescence staining: 5~10 g/mL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol
    Preservative
    Azide free
    Storage
    -20 °C/-80 °C
    Storage Comment
    -20 C (For long term storage: -80 C)
  • Miyamoto, Hirata, Yamazaki, Kageyama, Hasuwa, Mizushima, Tanaka, Yagi, Sonoda, Kai, Kanoh, Nakano, Mekada: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." in: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).

    Prenzel, Zwick, Daub, Leserer, Abraham, Wallasch, Ullrich: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." in: Nature, Vol. 402, Issue 6764, pp. 884-8, (2000) (PubMed).

    Iwamoto, Higashiyama, Mitamura, Taniguchi, Klagsbrun, Mekada et al.: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." in: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, (1994) (PubMed).

    Higashiyama, Abraham, Miller, Fiddes, Klagsbrun: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." in: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, (1991) (PubMed).

  • Target
    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
    Alternative Name
    HB-EGF (HBEGF Products)
    Synonyms
    hbegf antibody, zgc:165443 antibody, HBEGF antibody, zgc:175091 antibody, DTR antibody, DTS antibody, DTSF antibody, HEGFL antibody, Dtr antibody, GFHB antibody, Hb-egf antibody, Hegfl antibody, AW047313 antibody, Dts antibody, HB-EGF antibody, heparin-binding EGF-like growth factor b antibody, heparin binding EGF like growth factor antibody, Heparin-binding EGF-like growth factor antibody, heparin-binding EGF-like growth factor a antibody, heparin-binding EGF-like growth factor antibody, hbegfb antibody, HBEGF antibody, hbegf antibody, hbegfa antibody, Hbegf antibody
    Background
    Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratory of Prof. E. Mekada of Osaka University, who is a leading scientist in this field.
    UniProt
    Q99075
    Pathways
    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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