CD40 antibody (Extracellular Domain)

Catalog No. ABIN2451933

Product Details anti-CD40 Antibody

Target: CD40

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Stimulation (St)

Purification: Purified from serum-free medium by salting-out and ion-exchange chromatography under mild conditions.

Sterility: Sterile filtered

Immunogen: Recombinant extracellular domain of CD40 Product, Purified from serum-free medium by salting-out and ion-exchange chromatography under mild conditions.

Clone: 5C3

Isotype: IgG1 kappa

Application Details

Application Notes: 1. Flowcytometry
2. Immunohistochemistry (acetone or zinc-fixed or frozen section, indirect immuno-staining)
3. Stimulation of proliferation of B cell and dendritic cells. Enhancement of proinflamentory cytokine production in human monocytes,such as TNF-a,IL-6 and IL-8

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol. Azide- and carrier protein-free.

Preservative: Azide free

Storage: -20 °C

References for anti-CD40 antibody (ABIN2451933)

Ishida, Kumanogoh, Suzuki, Akahani, Noda, Kikutani: "Involvement of CD100, a lymphocyte semaphorin, in the activation of the human immune system via CD72: implications for the regulation of immune and inflammatory responses." in: International immunology, Vol. 15, Issue 8, pp. 1027-34, (2003) (PubMed).

Yasui, Muraoka, Takaoka-Shichijo, Ishida, Takegahara, Uchida, Kumanogoh, Suematsu, Suzuki, Kikutani: "Dissection of B cell differentiation during primary immune responses in mice with altered CD40 signals." in: International immunology, Vol. 14, Issue 3, pp. 319-29, (2002) (PubMed).

Inui, Kaisho, Kikutani, Stamenkovic, Seed, Clark, Kishimoto: "Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40." in: European journal of immunology, Vol. 20, Issue 8, pp. 1747-53, (1990) (PubMed).

Target Details for CD40

Target: CD40

Alternative Name: CD40 (CD40 Products)

Synonyms: Bp50 antibody, CDW40 antibody, TNFRSF5 antibody, p50 antibody, AI326936 antibody, GP39 antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TRAP antibody, Tnfrsf5 antibody, TNFSF5 antibody, CD40 molecule antibody, CD40 antigen antibody, CD40 antibody, Cd40 antibody

Background: CD40 is a 45-50- kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells, playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation, differentiation, immunoglobulin isotype switching, germinal center formation, and stimulation of the humoral memory response. CD40 has been found to mediate a broad variety of immune and inflammatory responses. Within the cell, the CD40 Molecule acts as a transmembrane signal transducer that leads to activation of intracellular kinases and transcription factors.

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints