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GFP antibody (Biotin)

GFP Reactivity: Aequorea victoria WB, ELISA, IF, IHC Host: Mouse Monoclonal 1002 Biotin

Catalog No. ABIN243774

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
174

15

11

9
Aequorea victoria
Host
63

63

34

13

9

5

2

1
Mouse
Compatible Secondaries
Clonality
113

72
Monoclonal
Conjugate
82

11

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Biotin
Application
147

79

46

33

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificity

Recognizes wild type and recombinant forms of Green Fluorescent Protein (GFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-mouse serum. Does not recognize enhanced forms of GFP.

Purification

Protein A purified

Immunogen

GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Clone

1002

Isotype

IgG1 kappa

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GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: ELISA (1:10000), IF, IHC (1:1000 - 1:3000), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:10000 (sandwich or capture). Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:1000-1:3000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Aequorea victoria

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Name/Gene ID: GFP

Synonyms: GFP

UniProt

P42212