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GFP antibody (Alkaline Phosphatase (AP))

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Mouse Monoclonal Alkaline Phosphatase (AP)

Catalog No. ABIN243771

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
174

15

11

9
Aequorea victoria
Host
63

63

34

13

9

5

2

1
Mouse
Compatible Secondaries
Clonality
113

72
Monoclonal
Conjugate
82

12

11

10

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Alkaline Phosphatase (AP)
Application
147

79

47

33

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

Recognizes wild type, recombinant and enhanced forms of GFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Purification

Protein A purified

Immunogen

GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Isotype

IgG1 kappa

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Application Notes

Approved: ELISA (1:1000 - 1:5000), IHC, WB

Usage: Suitable for use in ELISA (sandwich or capture), Western Blot and Immunohistochemistry. in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Aequorea victoria

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol, 10 mg/mL BSA, (IgG, protease free), 0.01 % sodium azide,  pH 8.0.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

avoid freezing

Storage

4 °C

Storage Comment

4°C, avoid freezing.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Name/Gene ID: GFP

Synonyms: GFP

UniProt

P42212