PC antibody (Internal Region)

Catalog No. ABIN238663

Product Details anti-PC Antibody

Target: PC (Pyruvate Carboxylase (PC))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: Pyruvate Carboxylase

Sequence: KFKEVKKAYV EANQ

Specificity: All reported variants (NP_000911.2, NP_001035806.1, NP_071504.2) represent identical protein.

Predicted Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-KFKEVKKAYVEANQ

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 150 kDa band observed in Mouse Liver lysates (calculated MW of 130 kDa according to NP_000911.2, NP_001035806.1 and NP_071504.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: Pyruvate Carboxylase (PC Products)

Background: PC, pyruvate carboxylase, PCB

Gene ID: 5091

NCBI Accession: NP_000911, NP_001035806, NP_071504