LASP1 antibody (Internal Region)

Catalog No. ABIN238605

Product Details anti-LASP1 Antibody

Target: LASP1 (LIM and SH3 Protein 1 (LASP1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LASP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: LASP1 (internal region)

Sequence: PERRDSQDGS SYR

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-PERRDSQDGSSYR, from the internal region of the protein sequence according to NP_006139.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon and Small Intestine. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 36 kDa band observed in Human Kidney lysates (calculated MW of 29.7 kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LASP1

Target: LASP1 (LIM and SH3 Protein 1 (LASP1))

Alternative Name: LASP1 (LASP1 Products)

Background: LASP1, LIM and SH3 protein 1, Lasp-1, MLN50

Gene ID: 3927

NCBI Accession: NP_006139