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DIAPH3 antibody (Internal Region)

Recent DIAPH3 Reactivity: Human ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN238601
  • Target See all DIAPH3 Antibodies
    DIAPH3 (Diaphanous Homolog 3 (Drosophila) (DIAPH3))
    Binding Specificity
    • 15
    • 9
    • 9
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 43
    • 17
    • 9
    Human
    Host
    • 54
    • 4
    Goat
    Clonality
    • 56
    • 2
    Polyclonal
    Conjugate
    • 23
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DIAPH3 antibody is un-conjugated
    Application
    • 45
    • 23
    • 13
    • 13
    • 6
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    ELISA
    Purpose
    DIAPH3
    Sequence
    RTPMPKDVRQ S
    Specificity
    This antibody is expected to recognise both reported isoforms (NP_001035982.1 and NP_112194.2).
    Predicted Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Recent
    Immunogen
    Peptide with sequence C-RTPMPKDVRQS, from the internal region (near the C terminus) of the protein sequence according to NP_001035982.1, NP_112194.2.
    Isotype
    IgG
    Top Product
    Discover our top product DIAPH3 Primary Antibody
  • Application Notes

    DS WB Results: Preliminary experiments gave an approx 50 kDa band in human bone marrow, liver and lymph node lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137 kDa according to NP_001035982.1 and 98.1 kDa according to NP_112194.2. The ~50 kDa band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    DIAPH3 (Diaphanous Homolog 3 (Drosophila) (DIAPH3))
    Alternative Name
    DIAPH3 (DIAPH3 Products)
    Synonyms
    Diap3 antibody, DIAP3 antibody, DIAPH3 antibody, 4930417P13Rik antibody, Dia2 antibody, Diaph3 antibody, Drf3 antibody, mDia2 antibody, mKIAA4117 antibody, p134MDia2 antibody, AN antibody, AUNA1 antibody, DIA2 antibody, DRF3 antibody, NSDAN antibody, diap3 antibody, diaphanous-related formin 3 antibody, diaphanous related formin 3 antibody, diaphanous homolog 3 (Drosophila) antibody, Diaph3 antibody, DIAPH3 antibody
    Background
    DIAPH3, diaphanous homolog 3 (Drosophila), RP11-26P21.1, DKFZP434C0931, DKFZp686A13178, DRF3, FLJ34705, diap3, diaphanous homolog 3, diaphanous-related formin 3
    Gene ID
    81624
    NCBI Accession
    NP_001035982, NP_112194
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