EPB41L2 antibody (Internal Region)

Catalog No. ABIN238594

Product Details anti-EPB41L2 Antibody

Target: EPB41L2 (erythrocyte Membrane Protein Band 4.1-Like 2 (EPB41L2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EPB41L2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: EPB41L2 / 4.1G (aa 593 to 604)

Sequence: RREVRSPTKA PH

Specificity: This antibody is reported to recognise all isoforms NP_001422.1 (iso a), NP_001129026.1 (iso b), NP_001186317.1 (iso c), NP_001239589.1 (iso d), NP_001337228.1 (iso e), NP_001337230.1 (iso f), NP_001337232.1 (iso g),NP_001337233.1 (iso h), NP_001337236.1

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RREVRSPTKAPH, from the internal region of the protein sequence according to NP_001422.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus and Colon. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary testing showed bands at approx 110+40 kDa in Human Cerebellum lysatesafter 0.1 μg/mL antibody staining (calculated MW of 113 kDa according to NP_001422.1 These bands were successfully blocked by incubation with the immunizing pept

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and in the cell junctions of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Re

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EPB41L2

Target: EPB41L2 (erythrocyte Membrane Protein Band 4.1-Like 2 (EPB41L2))

Alternative Name: EPB41L2 (EPB41L2 Products)

Synonyms: EPB41L2 antibody, 4.1-G antibody, 4.1G antibody, RGD1563977 antibody, AW555191 antibody, D10Ertd398e antibody, Epb41l2 antibody, NBL2 antibody, erythrocyte membrane protein band 4.1 like 2 antibody, erythrocyte membrane protein band 4.1-like 2 antibody, wu:fi46h04 antibody, EPB41L2 antibody, epb41l2 antibody, wu:fi46h04 antibody, Epb41l2 antibody

Background: EPB41L2, 4.1-G, erythrocyte membrane protein band 4.1-like 2, RP3-324N14.1, DKFZp781D1972, DKFZp781H1755, OTTHUMP00000040262, erythrocyte membrane protein band 4.1 like-protein 2

Gene ID: 2037

NCBI Accession: NP_001422