PTBP2 antibody (Internal Region)

Catalog No. ABIN238583

Product Details anti-PTBP2 Antibody

Target: PTBP2 (Polypyrimidine Tract Binding Protein 2 (PTBP2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PTBP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: PTBP2

Sequence: SKKFKGEDKM D

Specificity: This antibody is expected to recognise NP_001287914.1 (isoform 1), NP_001287915.1 (isoform 2), NP_001287916.1 (isoform 3), NP_001287917.1 (isoform 4), NP_001287918.1 (isoform 5), and NP_067013.1 (isoform 6).

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SKKFKGEDKMD, from the internal region of the protein sequence according to NP_067013.1 .

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55-60 kDa band observed in Mouse Testis lysates and in nuclear lysates of cell line NIH3T3, and approx 55 kDa in preliminary testing at Rat testes lysate (calculated MW of 57.6 kDa according to Mouse NP_062423.1 and 57.5 kDa according t

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PTBP2

Target: PTBP2 (Polypyrimidine Tract Binding Protein 2 (PTBP2))

Alternative Name: PTBP2 (PTBP2 Products)

Background: PTBP2, polypyrimidine tract binding protein 2, FLJ34897, MIBP, PTB, PTBLP, brPTB, nPTB, nPTB5, nPTB6, nPTB7, nPTB8, PTB-like, neural polypyrimidine tract binding protein, splicing regulator

Gene ID: 58155, 56195, 310820

NCBI Accession: NP_067013

Pathways: Ribonucleoprotein Complex Subunit Organization, SARS-CoV-2 Protein Interactome