CK1 epsilon antibody (C-Term)

Catalog No. ABIN238540

Product Details anti-CK1 epsilon Antibody

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CK1 epsilon antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: CSNK1E

Sequence: PASQTSVPFD HLGK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1, NP_689407.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45 kDa in nuclear lysates of cell line K562 (calculated MW of 47.3 kDa according to NP_001276841.1). Recommended concentration: 0.3-1 μg/mL. Primary incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Alternative Name: CSNK1E (CSNK1E Products)

Synonyms: CKIepsilon antibody, HCKIE antibody, CKIe antibody, ck1-epsilon antibody, ck1epsilon antibody, hckie antibody, dco antibody, fj38c01 antibody, wu:fj38c01 antibody, wu:fj62a04 antibody, zgc:77310 antibody, AI426939 antibody, AI551861 antibody, AW457082 antibody, CK1epsilon antibody, KC1epsilon antibody, tau antibody, ckie antibody, CSNK1E antibody, Csnk1e antibody, CKI-epsilon antibody, 0538/13 antibody, 0915/10 antibody, 1396/02 antibody, 1447/01 antibody, 1460/09 antibody, CG2048 antibody, CK1delta/epsilon antibody, DBT antibody, DBT/CK1epsilon antibody, DCO antibody, DCO/CKIe antibody, Dbt antibody, Dco antibody, Dco/CK1 antibody, Dmel\CG2048 antibody, dCKIepsilon antibody, dbt antibody, dco-1 antibody, ddbt antibody, l(3)S053813 antibody, l(3)S144701 antibody, l(3)dco antibody, l(3)dco-1 antibody, l(3)discs overgrown antibody, l(3)discs overgrown-1 antibody, l(3)j3B9 antibody, l(3)rK215 antibody, casein kinase 1 epsilon antibody, casein kinase 1, epsilon antibody, casein kinase 1 epsilon L homeolog antibody, casein kinase I isoform epsilon antibody, casein kinase I epsilon antibody, casein kinase I antibody, LOC400927-CSNK1E readthrough antibody, discs overgrown antibody, CSNK1E antibody, csnk1e antibody, Csnk1e antibody, csnk1e.L antibody, LOC443240 antibody, NAEGRDRAFT_82890 antibody, LOC100407384 antibody, LOC100523244 antibody, LOC100607246 antibody, LOC100727500 antibody, LOC400927-CSNK1E antibody, dco antibody

Background: CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon

Gene ID: 1454, 27373, 58822

NCBI Accession: NP_001276841

Pathways: Hedgehog Signaling, M Phase