HLA-ABC antibody (FITC)

Catalog No. ABIN238526

Product Details anti-HLA-ABC Antibody (FITC)

Target: HLA-ABC

Reactivity: Human, Cow, Cat, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-ABC antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Characteristics: Synonyms: HLA-ABC, HLA class I histocompatibility antigen, MHC class I antigen

Immunogen: Membrane of human tonsil cells. Remarks: Gene Locus: 6p21. 3

Clone: W6-32

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry (starting dilution 1/300).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-HLA-ABC antibody (FITC) (ABIN238526)

Plánka, Necas, Srnec, Rauser, Starý, Jancár, Amler, Filová, Hlucilová, Kren, Gál: "Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 6, pp. 885-93, (2010) (PubMed).

Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).

Drosopoulos, Roberts, Cermak, Sasazuki, Shirasawa, Andera, Pintzas: "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22856-67, (2005) (PubMed).

Le Discorde, Moreau, Sabatier, Legeais, Carosella: "Expression of HLA-G in human cornea, an immune-privileged tissue." in: Human immunology, Vol. 64, Issue 11, pp. 1039-44, (2003) (PubMed).

Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." in: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).

Ladasky, Shum, Canavez, Seuánez, Parham: "Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody." in: Immunogenetics, Vol. 49, Issue 4, pp. 312-20, (1999) (PubMed).

Shields, Ribaudo: "Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin." in: Tissue antigens, Vol. 51, Issue 5, pp. 567-70, (1998) (PubMed).

Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, (1994) (PubMed).

Kievits, Ivanyi: "Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin." in: Human immunology, Vol. 20, Issue 2, pp. 115-26, (1988) (PubMed).

Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, (1987) (PubMed).

Neefjes, Hensen, de Kroon, Ploegh: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." in: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, (1986) (PubMed).

Brodsky, Parham: "Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies." in: Immunogenetics, Vol. 15, Issue 2, pp. 151-66, (1982) (PubMed).

Target Details for HLA-ABC

Target: HLA-ABC

Alternative Name: HLA Class I ABC (HLA-ABC Products)

Synonyms: HLAA antibody, major histocompatibility complex, class I, A antibody, HLA-A antibody

Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta-2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.Synonyms: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints