Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% acetic acid and subsequently used forimmunization.
CLU
Reactivity: Human, Rat, Mouse
WB, IHC, ELISA, ICC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA. Western blot: 1/ μg/mLPositive Material: seminal plasmaImmunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 μg/mL. Positive Tissue: Sertoli cells.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH ~7.4 with 15 mM Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2