p53 antibody (C-Term, pSer392)

Catalog No. ABIN238404

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: AA 378-393, C-Term, pSer392

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This p53 antibody FP3.2 [FPS392] reacts with Human p53 tumour suppressor protein phosphorylated at CKII site (Ser392).

Cross-Reactivity (Details): Species reactivity (expected):Rabbit (85 %), Canine (85 %), Guinea pig (85 %), Monkey (92 %), Pig (85 %).
Species reactivity (tested):Human, Mouse.

Characteristics: Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13

Purification: Protein A Affinity Chromatography

Immunogen: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P] corresponding to amino acids378-393 representing the C-terminus of Human p53.

Clone: FP3-2

Isotype: IgG1

Application Details

Application Notes: Western Blotting: 1 μg/mL. Immunohistochemistry on Paraffin Sections): 10 μg/mLStaining technique: Standard ABC technique (DAB+)Pretreatment: High temperature antigen retrieval (microwave, pressure cooker) in 10 mMcitrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0. Incubation Time: 1 hour at RT or overnight at 4 °CPositive Tissue: breast carcinoma with high level of wild-type p53.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53 (TP53) (TP53 Products)

Background: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Gene ID: 7157

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect