CEBPD antibody (N-Term)

Catalog No. ABIN233849

Supplier Product No.: 600-401-a61

Product Details anti-CEBPD Antibody

Target: CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))

Binding Specificity: N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-a61

Supplier: Rockland

Purpose: CEBP Delta Antibody

Cross-Reactivity (Details): This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein.

Characteristics: Synonyms: rabbit anti-CEBP Delta Antibody, C/EBP related protein 3 antibody, CCAAT/enhancer binding protein delta antibody, CEBP D antibody, CEBPD antibody, CELF antibody, Crp 3 antibody, Crp3 antibody, NF IL6 beta antibody, Nuclear factor NF IL6 beta antibody

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:2000

Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting, and suitable in IHC.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract.   The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.

Western Blot Dilution: 1:1,000 - 1:15,000

Immunoprecipitation Dilution: 0.8 - 1.5 μg/mL

ELISA Dilution: 1:4,000 - 1:9,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.83 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CEBPD antibody (ABIN233849)

Puzio-Kuter, Laddha, Castillo-Martin, Sun, Cordon-Cardo, Chan, Levine: "Involvement of tumor suppressors PTEN and p53 in the formation of multiple subtypes of liposarcoma." in: Cell death and differentiation, Vol. 22, Issue 11, pp. 1785-91, (2015) (PubMed).

Sarkar, Sharan, Wang, Pawar, Cantwell, Johnson, Morrison, Wang, Sterneck: "Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells." in: Molecular and cellular biology, Vol. 32, Issue 2, pp. 320-32, (2012) (PubMed).

Balamurugan, Wang, Tsai, Sharan, Anver, Leighty, Sterneck: "The tumour suppressor C/EBPδ inhibits FBXW7 expression and promotes mammary tumour metastasis." in: The EMBO journal, Vol. 29, Issue 24, pp. 4106-17, (2011) (PubMed).

Target Details for CEBPD

Target: CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))

Alternative Name: Cebpd (CEBPD Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.  Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression.  Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T).  Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity.  C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Gene ID: 12609, 110347410

UniProt: Q00322