IL17RA antibody

Catalog No. ABIN233847

Supplier Product No.: 600-401-a49

Product Details anti-IL17RA Antibody

Target: IL17RA (Interleukin 17 Receptor A (IL17RA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17RA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-a49

Supplier: Rockland

Purpose: IL-7 Receptor Alpha Chain phospho Y449 Antibody

Cross-Reactivity (Details): This antibody is specific for mouse IL-7 protein phosphorylated at Y449.

Characteristics: Synonyms: rabbit anti-IL-7 Receptor alpha pY449 antibody, rabbit anti-Interleukin-7 Receptor alpha pY449 antibody, IL7R alpha antibody, IL-7R alpha, IL 7R α, Interleukin7 Receptor, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor α antibody

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against non-phospho-peptide.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 55 kDa in size corresponding to phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:10,000

ELISA Dilution: 1:5,000-1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.82 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store IL-7 Receptor Alpha Chain phospho Y449 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IL17RA antibody (ABIN233847)

Osborne, Dhanji, Snow, Priatel, Ma, Miners, Teh, Goldsmith, Abraham: "Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice." in: The Journal of experimental medicine, Vol. 204, Issue 3, pp. 619-31, (2007) (PubMed).

Target Details for IL17RA

Target: IL17RA (Interleukin 17 Receptor A (IL17RA))

Alternative Name: IL17R (IL17RA Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R), the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.  The human and mouse sequence is nearly identical.  In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. IL-7 Receptor Alpha Chain phospho Y449 Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Immunology research.

Gene ID: 16197, 45269152

UniProt: P16872

Pathways: SARS-CoV-2 Protein Interactome