SIPA1 antibody (N-Term)

Catalog No. ABIN233836

Supplier Product No.: 600-401-a36

Product Details anti-SIPA1 Antibody

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIPA1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 600-401-a36

Supplier: Rockland

Purpose: Sipa1 Antibody

Cross-Reactivity (Details): This antibody is specific for mouse Sipa1 protein.

Characteristics: Synonyms: rabbit anti-Sipa1 antibody, Sipa-1, Sipa 1, GTPase activating protein Spa 1 antibody, p130 SPA-1 antibody, Signal induced proliferation associated 1 antibody, SPA1

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1.25-2.5 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 130 kDa in size corresponding

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.25 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SIPA1

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Alternative Name: Sipa1 (SIPA1 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Sipa1 (signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. Sipa1 is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.

Gene ID: 20469

UniProt: P46062

Pathways: Response to Water Deprivation