ATR antibody (Internal Region)

Catalog No. ABIN233834

Supplier Product No.: 600-401-a30

Product Details anti-ATR Antibody

Target: ATR (ATR serine/threonine kinase (ATR))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATR antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 600-401-a30

Supplier: Rockland

Purpose: ATR Antibody

Cross-Reactivity (Details): This antibody is specific for human ATR protein.

Characteristics: Synonyms: rabbit anti-ATR antibody, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, ACTR antibody, AIB1 antibody, amplified in breast cancer 1 antibody, CAGH16 antibody, CBP interacting protein antibody, CTG26 antibody, FRP1, FRP-1, FRP 1

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 301 kDa in size corresponding to ATR by western blotting in the appropriate cell lysate or extract.  Lysates from HeLa, U2OS, RAW264.7 or HEK293 cells are suggested for western blotting.  ATR is ubiquitously expressed, with highest expression levels in the testes.  Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:15,000 - 1:70,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.28 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ATR

Target: ATR (ATR serine/threonine kinase (ATR))

Alternative Name: ATR (ATR Products)

Background: Background: Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidyl-inositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle.  ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor.  ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4).  ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis.  ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types.  ATR is recruited to chromatin during S-phase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

Gene ID: 545, 157266317

UniProt: Q13535

Pathways: Positive Regulation of Response to DNA Damage Stimulus