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RSL1D1 antibody

Rockland 600-401-a24 RSL1D1 Reactivity: Human WB, ELISA, IF, IP, FM Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN233832
Supplier Product No.: 600-401-a24
  • Target See all RSL1D1 products
    RSL1D1 (Ribosomal L1 Domain Containing 1 (RSL1D1))
    Reactivity
    • 7
    • 4
    • 3
    Human
    Host
    • 7
    • 2
    • 1
    Rabbit
    Clonality
    • 10
    Polyclonal
    Conjugate
    • 8
    • 1
    • 1
    This RSL1D1 antibody is un-conjugated
    Application
    • 10
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-a24
    Supplier
    Rockland
    Purpose
    PBK1 Antibody
    Cross-Reactivity (Details)
    This antibody is specific for human PBK1 protein.
    Characteristics
    Synonyms: rabbit anti-PBK1 antibody, PBK-1, PBK 1, CATX-11, CATX11 antibody, RSL1D1, Cellular senescence inhibited gene protein antibody, CSIG antibody, L12 antibody, Ribosomal L1 domain containing protein 1 antibody
    Purification
    This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
  • Application Notes

    Application Note: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 55 kDa in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract.  This antibody is capable of detecting both over-expressed and endogenous PBK1. For immunofluorescence microscopy, fix cells with methanol.

    Western Blot Dilution: 1:500 - 1:2,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:2,500 - 1:10,000

    IF Microscopy Dilution: 1:100

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    RSL1D1 (Ribosomal L1 Domain Containing 1 (RSL1D1))
    Alternative Name
    RSL1D1 (RSL1D1 Products)
    Background
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). PBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004).  A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and activation of other protease systems involved in cell invasion (Tong et al., 2005). The cellular localization dynamics of PBK1, as well as the data from its yeast homologue (Cic1p/Nsa3p) suggest that PBK1 is essential for ribosome biogenesis (Fatica et al., 2003). Thus, PBK1 could be controlling cell proliferation by regulating the level of ribosome production. It is hypothesized that PBK1 is involved in regulating both, cell proliferation and invasiveness, therefore playing a dual function in cancer cells transformation.
    Gene ID
    26156, 62901068
    UniProt
    O76021
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