PPP1R13B antibody

Catalog No. ABIN233830

Supplier Product No.: 600-401-a18

Product Details anti-PPP1R13B Antibody

Target: PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R13B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-a18

Supplier: Rockland

Purpose: ASPP1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human ASPP1.

Characteristics: Synonyms: rabbit anti-ASPP1 Antibody, ASPP-1, ASPP 1, p53BP2 like antibody, p85 antibody, PPP1R13B antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 120 kDa in size corresponding to ASPP1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:2,500 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PPP1R13B

Target: PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))

Alternative Name: PPP1R13B (PPP1R13B Products)

Background: Background: ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins.  ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis.  In contrast, iASPP functionally inactivates p53.  ASPPs may also regulate p63- and p73-mediated apoptosis.  Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3.  Not all cell cycle arrest genes are affected, such as p21.  Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53.  Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.

Gene ID: 23368

NCBI Accession: NP_056131

UniProt: Q96KQ4

Pathways: p53 Signaling