ROCK2 antibody (pTyr256)

Catalog No. ABIN233829

Product Details anti-ROCK2 Antibody

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Binding Specificity: pTyr256

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human ROCK-2 pY256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-ROCK2 antibody (ABIN233829)

Akhtar, Yousif, Dhaunsi, Sarkhouh, Chandrasekhar, Attur, Benter: "Activation of ErbB2 and Downstream Signalling via Rho Kinases and ERK1/2 Contributes to Diabetes-Induced Vascular Dysfunction." in: PLoS ONE, Vol. 8, Issue 6, pp. e67813, (2017) (PubMed).

Target Details for ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: Rock-2 (ROCK2 Products)

Synonyms: rocka-a antibody, xROK antibody, rock2 antibody, rok2 antibody, ROCK2 antibody, B230113H15Rik antibody, ROKalpha antibody, Rho-kinase antibody, Rock-II antibody, Rock2m antibody, mKIAA0619 antibody, ROCK-II antibody, ROK antibody, Rho-associated, coiled-coil containing protein kinase 2 L homeolog antibody, rho-associated, coiled-coil containing protein kinase 2a antibody, Rho associated coiled-coil containing protein kinase 2 antibody, Rho-associated, coiled-coil containing protein kinase 2 antibody, Rho-associated coiled-coil containing protein kinase 2 antibody, rock2.L antibody, rock2a antibody, ROCK2 antibody, Rock2 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases.  Increased levels of RhoA or RhoC, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.
Synonyms: Rho-associated protein kinase 2 EC=2.7.11.1 Rho-associated, coiled-coil-containing protein kinase 2 p164 ROCK-2 Rho kinase 2

Gene ID: 9475, 4520225

UniProt: O75116

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation