MYO1G antibody (C-Term)

Catalog No. ABIN233814

Supplier Product No.: 600-401-959

Product Details anti-MYO1G Antibody

Target: MYO1G (Myosin IG (MYO1G))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYO1G antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-959

Supplier: Rockland

Purpose: Myosin 1G Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human Myosin 1G protein.

Characteristics: Synonyms: rabbit anti-Myosin 1G antibody, Unconventional myosin-Ig, Minor histocompatibility antigen HA-2, mHag HA-2, MYO1G

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human Myosin 1G protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA, western blotting and possibly in immunoprecipitation as well.  Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 100 kDa in size corresponding to Myosin 1G protein is expected in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-MYO1G antibody (ABIN233814)

Olety, Wälte, Honnert, Schillers, Bähler: "Myosin 1G (Myo1G) is a haematopoietic specific myosin that localises to the plasma membrane and regulates cell elasticity." in: FEBS letters, Vol. 584, Issue 3, pp. 493-9, (2010) (PubMed).

Target Details for MYO1G

Target: MYO1G (Myosin IG (MYO1G))

Alternative Name: MYO1G (MYO1G Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. In general, myosins are protein complexes consisting of one or more myosin heavy chains, associated light chains and other proteins.  Myosins function as molecular motors and use the energy of ATP hydrolysis to move actin filaments or to move vesicles or other cargo on fixed actin filaments.  Myosins have magnesium-ATPase activity and bind actin. Myosins can be divided into classes that are distinguished based on sequence features of the motor, or head domain, but also have distinct tail regions that are believed to bind specific cargoes. Unconventional myosins exist.  Myosin 1G is an unconventional myosin that is restricted to hematopoietic cells. Unconventional myosins are also critical for motility in amoeba and a mammalian paralog (Myo1C) is critical as a glucose transporter that recycles glucose in response to insulin.

Gene ID: 64005, 239582755

UniProt: B0I1T2