TMBIM1 antibody (N-Term)

Catalog No. ABIN233805

Supplier Product No.: 200-401-a57

Product Details anti-TMBIM1 Antibody

Target: TMBIM1 (Transmembrane BAX Inhibitor Motif Containing 1 (TMBIM1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMBIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 200-401-a57

Supplier: Rockland

Purpose: TMBIM1 Antibody

Cross-Reactivity (Details): This antibody is specific for human TMBIM1 protein.

Characteristics: Synonyms: rabbit anti-TMBIM1 Antibody, Putative uncharacterized protein TMBIM1, Protein lifeguard 3, Protein RECS1 homolog, Transmembrane BAX inhibitor motif-containing protein 1, LFG3, RECS1, PP1201, PSEC0158

Purification: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of human TMBIM1 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 10 μg/mL

Application Note: This protein A purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to TMBIM-1 by western blotting in the appropriate cell lysate or extract.  To date this antibody has shown the ability to recognize over-expressed TMBIM1 but not endogenous protein.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-TMBIM1 antibody (ABIN233805)

Shukla, Fujita, Xiao, Liao, Garfield, Srinivasula: "A shear stress responsive gene product PP1201 protects against Fas-mediated apoptosis by reducing Fas expression on the cell surface." in: Apoptosis : an international journal on programmed cell death, (2010) (PubMed).

Target Details for TMBIM1

Target: TMBIM1 (Transmembrane BAX Inhibitor Motif Containing 1 (TMBIM1))

Alternative Name: TMBIM1 (TMBIM1 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. TMBIM1 (Transmembrane BAX inhibitor motif-containing protein 1) is a member of the 7 TMS (7 transmembrane domains) family of receptors known to mediate the activation of various transcription factors. TMBIM1 is identified as a novel modulator of NF-kB activation.  A drosophila homolog of this protein (dNMDA1 with 40 % homology to hTMBIM1) is reported to be unregulated during aging and oxidative stress.

Gene ID: 64114, 50593008

UniProt: Q969X1