SEPHS2 antibody

Catalog No. ABIN233801

Supplier Product No.: 200-401-999

Product Details anti-SEPHS2 Antibody

Target: SEPHS2 (Selenophosphate Synthetase 2 (SEPHS2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEPHS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-401-999

Supplier: Rockland

Purpose: Selenophosphate Synthetase 2 Antibody

Cross-Reactivity (Details): This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1.

Characteristics: Synonyms: rabbit anti-Selenophosphate Synthetase 2 Antibody, rabbit anti-SPS 2 antibody, Selenide water dikinase 2 antibody, Selenium donor protein 2 antibody, SEPHS2 antibody

Purification: This product was purified by Protein A chromatography from monospecific antiserum.

Sterility: Sterile filtered

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This Protein A purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 48 kDa in size corresponding to SPS2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-SEPHS2 antibody (ABIN233801)

Pitts, Kremer, Hashimoto, Torres, Byrns, Williams, Berry: "Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 46, pp. 15326-38, (2015) (PubMed).

Target Details for SEPHS2

Target: SEPHS2 (Selenophosphate Synthetase 2 (SEPHS2))

Alternative Name: Sephs2 (SEPHS2 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase (SelD) catalyzes the conversion of selenium to selenophosphate which is required by a number of bacterial, archaeal and eukaryotic organisms for synthesis of selenocysteine-tRNA, the precursor of selenocysteine in selenoenzymes.  A second selenophosphate synthetase (SPS2) was identified in mammals. SPS2 is itself a selenoprotein in mammals.

Gene ID: 20768, 14717785

UniProt: P97364