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p53 antibody (pSer20)

TP53 Reactivity: Human, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN229019

Datasheet as PDF

Target See all p53 (TP53) Antibodies

p53 (TP53) (Tumor Protein P53 (TP53))
Binding Specificity
43

40

37

30

29

29

26

25

24

22

20

20

19

19

18

18

16

15

13

9

8

8

8

7

7

6

6

6

6

6

6

6

5

5

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3
pSer20
Reactivity
823

251

208

104

47

33

31

18

10

9

7

6

6

3

3

3

2

2

2

2

2

1

1

1
Human, Mouse
Host
622

218

6

2

1
Rabbit
Compatible Secondaries
Clonality
558

290

1
Polyclonal
Conjugate
476

38

31

19

18

13

13

13

13

13

13

13

13

13

12

11

11

11

11

11

11

11

11

5

5

5

5

5

5

2

2

2

2

2

2

2

1

1

1

1

1

1
This p53 antibody is un-conjugated
Application
661

297

247

200

199

148

124

122

119

64

59

32

18

16

13

12

9

9

8

6

4

3

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), ELISA

Specificity

Detects endogenous levels of human p53 only when phosphorylated at serine 20. Does not detect p53 phosphorylated at other sites. Species cross-reactivity: Mouse.

Purification

Immunoaffinity purified

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser20 of human p53 (KLH).

Type of Immunogen: Synthetic phospho-peptide

Isotype

IgG

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anti-Tumor Protein P53 (TP53) (pSer33) antibody
TP53 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: ELISA, WB (1:1000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5 % BSA, 0.1 % Tween-20 at 4?C with gentle shaking, overnight.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative

Without preservative

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name

TP53 / p53 (TP53 Products)

Background

Name/Gene ID: TP53
Family: P53

Synonyms: TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1

Gene ID

7157

UniProt

P04637

Pathways

p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect