HRAS antibody (AA 62-76)
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- Target See all HRAS Antibodies
- HRAS (HRas proto-oncogene, GTPase (HRAS))
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Binding Specificity
- AA 62-76
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Reactivity
- Human, Mouse, Rat
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This HRAS antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Neutralization (Neut)
- Specificity
- Recognizes phosphorylated and non-phosphorylated human, rat and mouse ras. Does not cross-react with the rap protein. Neutralizes the biological and biochemical activities by binding to residues E63, S65, A66, M67, Q70 and R73. Positive Controls: SW480 or Y1 cells (IF and IP), normal skin (IHC, frozen and paraffin), A431, Hela or ML-1 cells (WB).
- Purification
- Purified
- Immunogen
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Recombinant p21 ras protein. Epitope: aa62-76 of human ras.
Type of Immunogen: Recombinant protein - Isotype
- IgG1 kappa
- Top Product
- Discover our top product HRAS Primary Antibody
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- Application Notes
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Approved: IF (2.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, Neut, WB (2.5 μg/mL)
Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Immunofluorescence: 2.5 μg/mL. Western Blot (chemiluminescence detection): 2.5 μg/mL. Immunohistochemistry (Frozen): 5 μg/mL. Immunohistochemistry: 1-5 μg/mL. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize. Immunoprecipitation (autoradiographic detection): 1 μg/sample SW480 or Y1 cells. Use rabbit anti-rat IgG plus Protein A or goat anti-rat IgG plus Protein G. Protein A alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- HRAS (HRas proto-oncogene, GTPase (HRAS))
- Alternative Name
- HRAS / H-Ras (HRAS Products)
- Synonyms
- C-BAS/HAS antibody, C-H-RAS antibody, C-HA-RAS1 antibody, CTLO antibody, H-RASIDX antibody, HAMSV antibody, HRAS1 antibody, K-RAS antibody, N-RAS antibody, RASH1 antibody, hras antibody, zgc:110250 antibody, HRAS antibody, H-RAS antibody, c-H-ras antibody, H-Ras antibody, K-Ras antibody, hras1 antibody, rash1 antibody, ras antibody, N-Ras antibody, c-bas/has antibody, H-ras antibody, Ha-ras antibody, Harvey-ras antibody, Hras-1 antibody, Kras2 antibody, c-Ha-ras antibody, c-rasHa antibody, hrasl antibody, zgc:110734 antibody, HRas proto-oncogene, GTPase antibody, v-Ha-ras Harvey rat sarcoma viral oncogene homolog a antibody, neuroblastoma RAS viral (v-ras) oncogene homolog pseudogene antibody, Harvey rat sarcoma viral oncogene homolog L homeolog antibody, Harvey rat sarcoma viral oncogene homolog antibody, Harvey rat sarcoma virus oncogene antibody, NRAS proto-oncogene, GTPase antibody, -Ha-ras Harvey rat sarcoma viral oncogene homolog b antibody, HRAS antibody, hrasa antibody, LOC733587 antibody, Hras antibody, hras.L antibody, hras antibody, NRAS antibody, hrasb antibody
- Background
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Name/Gene ID: HRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806
Synonyms: HRAS, C-H-RAS, C-has/bas p21 protein, C-ras-Ki-2 activated oncogene, C-HA-RAS1, CTLO, H-ras, H-RASIDX, GTP- and GDP-binding peptide B, GTPase HRas, H-Ras-1, HAMSV, HRAS1, K-RAS, Ha-Ras, Ha-Ras1 proto-oncoprotein, Transforming protein p21, N-ras, p21ras, RASH1, C-BAS/HAS, C-ha-ras, p19 H-RasIDX protein - Gene ID
- 3265
- UniProt
- P01112
- Pathways
- p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling
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