NEDD8 antibody (N-Term)

Catalog No. ABIN221543

Product Details anti-NEDD8 Antibody

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Monkey, Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: Detects endogenous levels of both free and conjugated NEDD8 protein. The antibody does not cross-react with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 and ISG15. Species cross-reactivity: Bovine, human, monkey, mouse, rat and Xenopus.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH-coupled) corresponding to amino acids at the amino terminus of human NEDD8 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Flo (1:200), IF (1:200), IHC, IHC-P, IP (1:25), WB (1:1000)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Flow Cytometry. Western Blot 1:1000. Immunoprecipitation 1:25. Immunofluorescence (IF-IC) 1:200. Flow Cytometry: 1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol, less than 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: NEDD8 (NEDD8 Products)

Synonyms: NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody

Background: Name/Gene ID: NEDD8

Synonyms: NEDD8, NEDD-8, Neddylin, Ubiquitin-like protein Nedd8

Gene ID: 4738

UniProt: Q15843

Pathways: Ubiquitin Proteasome Pathway