PKC alpha antibody (pThr638, Thr641)
-
- Target See all PKC alpha (PKCa) Antibodies
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
-
Binding Specificity
- pThr638, Thr641
-
Reactivity
- Human, Mouse, Rat, Monkey
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This PKC alpha antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Specificity
- Detects human PKC alpha only when phosphorylated at Thr638 and human PKC beta II only when phosphorylated at Thr641. Reacts weakly with phosphorylated PKC beta I and gamma. Recognizes the active form of PKC. Species cross-reactivity: Monkey and mouse. Predicted to cross-react with rat based on sequence homology.
- Purification
- Protein A purified
- Immunogen
-
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr638 of human PKC alpha.
Type of Immunogen: Synthetic phospho-peptide - Isotype
- IgG
- Top Product
- Discover our top product PKCa Primary Antibody
-
-
- Application Notes
-
Approved: ELISA, IP (1:100), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:100. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
- Target
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
- Alternative Name
- PRKCA / PKC-Alpha (PKCa Products)
- Synonyms
- pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody
- Background
-
Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase
Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha - Gene ID
- 5578
- UniProt
- P17252
- Pathways
- WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
-