Caspase 3 antibody (N-Term)

Catalog No. ABIN219228

Product Details anti-Caspase 3 Antibody

Target: Caspase 3 (CASP3)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes cleaved human Caspase 3. Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Species cross-reactivity: mouse, bovine, and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding residues adjacent to Asp175 in N-terminus of human caspase-3 (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:200), ICC, IF, IHC, IHC-Fr (1:200), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Recommend Dilution: Western Blot: 1:1000. Immunohistochemistry (Frozen): 1:200. Immunocytochemistry (IF):1:200. Flow Cytometry: 1:200.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3 (CASP3 Products)

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation