Complement C4d antibody

Catalog No. ABIN2192222

Product Details anti-Complement C4d Antibody

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement C4d antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: 12D11

Application Details

Application Notes: It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Complement C4d antibody (ABIN2192222)

Zwirner, Felber, Burger, Bitter-Suermann, Riethmüller, Feucht: "Classical pathway of complement activation in mammalian kidneys." in: Immunology, Vol. 80, Issue 2, pp. 162-7, (1994) (PubMed).

Feucht, Schneeberger, Hillebrand, Burkhardt, Weiss, Riethmüller, Land, Albert: "Capillary deposition of C4d complement fragment and early renal graft loss." in: Kidney international, Vol. 43, Issue 6, pp. 1333-8, (1993) (PubMed).

Target Details for Complement C4d

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Alternative Name: c4d (C4d Products)

Background: Chain of human C4d. Complement factor C4 (MW 41 kDa), formerly known as Gg protein, consists of an alpha-, beta- and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. Activated C1, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved by factor I resulting in C4c and C4d, in which step all functional sites are lost. The C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. The thioester formed between the side chains of Cys1010 and Gln1013 within the C4d region of the α-chain mediates covalent attachment to the target surface bearing activated forms of C1s or MASP. Furthermore, C4d is highly homologous to C3d with over 35 % shared amino acid sequence. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies. The monoclonal antibody 12D11 recognizes C4, C4b and C4d. Antibody 12D11 cross reacts with pig, bovine, baboon and rhesus monkey. Mouse IgG1