Junctional Adhesion Molecule-A 2 (Extracellular Domain) antibody

Catalog No. ABIN2192198

Product Details

Target: Junctional Adhesion Molecule-A 2

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blot, flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Zernecke, Liehn, Fraemohs, von Hundelshausen, Koenen, Corada, Dejana, Weber: "Importance of junctional adhesion molecule-A for neointimal lesion formation and infiltration in atherosclerosis-prone mice." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 2, pp. e10-3, (2006) (PubMed).

Ostermann, Fraemohs, Baltus, Schober, Lietz, Zernecke, Liehn, Weber: "Involvement of JAM-A in mononuclear cell recruitment on inflamed or atherosclerotic endothelium: inhibition by soluble JAM-A." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 4, pp. 729-35, (2005) (PubMed).

Fraemohs, Koenen, Ostermann, Heinemann, Weber: "The functional interaction of the beta 2 integrin lymphocyte function-associated antigen-1 with junctional adhesion molecule-A is mediated by the I domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 10, pp. 6259-64, (2004) (PubMed).

Ostermann, Weber, Zernecke, Schröder, Weber: "JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes." in: Nature immunology, Vol. 3, Issue 2, pp. 151-8, (2002) (PubMed).

Target Details

Target: Junctional Adhesion Molecule-A 2

Background: The polyclonal antibody reacts with the second extracellular domain of human junction adhesion molecule (JAM)-A (also known as JAM, JAM-1 or F11R). Together with JAM-C (JAM-2) and JAM-B (VE- JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A play an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the b2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role of JAM-A in the initiation of atherosclerosis, since JAM-A is upregulated on early atherosclerotic endothelium and adhesion of activated platelets on activated endothelium is mediated by homophilic interactions of JAM- A. The polyclonal antibody reacts with the 17 kDa extracellular domain 2 of the human JAM-A protein. The immunogen used for rabbit immunization is the extracellular domain of full-length human JAM-A. The antibody does not react with mouse JAM-A.