Nitrotyrosine antibody

Catalog No. ABIN2192139

Product Details anti-Nitrotyrosine Antibody

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA)

Cross-Reactivity (Details): Cross reactivity: Phosphotyrosine : No, Chlorotyrosine : No

Sterility: 0.2 μm filtered

Clone: HM-11

Isotype: IgG2b

Application Details

Application Notes: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive W: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate control P: human lung tissue

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-Nitrotyrosine antibody (ABIN2192139)

Tsuhako, Augusto, Linares, Chadi, Giorgio, Pereira: "Tempol ameliorates murine viral encephalomyelitis by preserving the blood-brain barrier, reducing viral load, and lessening inflammation." in: Free radical biology & medicine, Vol. 48, Issue 5, pp. 704-12, (2010) (PubMed).

Han, Drabek, Stezoski, Janesko-Feldman, Stezoski, Clark, Bayir, Tisherman, Kochanek: "Protein nitration and poly-ADP-ribosylation in brain after rapid exsanguination cardiac arrest in a rat model of emergency preservation and resuscitation." in: Resuscitation, Vol. 79, Issue 2, pp. 301-10, (2008) (PubMed).

Casoni, Basso, Massignan, Gianazza, Cheroni, Salmona, Bendotti, Bonetto: "Protein nitration in a mouse model of familial amyotrophic lateral sclerosis: possible multifunctional role in the pathogenesis." in: The Journal of biological chemistry, Vol. 280, Issue 16, pp. 16295-304, (2005) (PubMed).

Klausz, Tiszai, Tiszlavicz, Gyulai, Lénárt, Lonovics, Mándi: "Local and peripheral cytokine response and CagA status of Helicobacter pylori-positive patients with duodenal ulcer." in: European cytokine network, Vol. 14, Issue 3, pp. 143-8, (2003) (PubMed).

ter Steege, Koster-Kamphuis, van Straaten, Forget, Buurman: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." in: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Abstract: Nitrotyrosine Products

Target Type: Chemical

Background: The monoclonal antibody HM.11 recognizes modified amino acid nitrotyrosine in all different species. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage. The monoclonal antibody HM.11 recognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. Immunogen Nitrated KLH