Selectin E/CD62e antibody

Catalog No. ABIN2192127

Product Details anti-Selectin E/CD62e Antibody

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunoassay (IA), Immunostaining (ISt)

Sterility: 0.2 μm filtered

Clone: ENA1

Application Details

Application Notes: For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1 % paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/mL), IL-1 (100 U/mL) or LPS (1 μg/mL) in tissue culture medium at 37 °C. As negative control it is advised to use a control murine IgG1 antibody.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Selectin E/CD62e antibody (ABIN2192127)

Leeuwenberg, Van de Winkel, Jeunhomme, Buurman: "Functional polymorphism of IgG FcRII (CD32) on human neutrophils." in: Immunology, Vol. 71, Issue 2, pp. 301-4, (1990) (PubMed).

Leeuwenberg, von Asmuth, Jeunhomme, Buurman: "IFN-gamma regulates the expression of the adhesion molecule ELAM-1 and IL-6 production by human endothelial cells in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 7, pp. 2110-4, (1990) (PubMed).

Leeuwenberg, Jeunhomme, Buurman: "Induction of an activation antigen on human endothelial cells in vitro." in: European journal of immunology, Vol. 19, Issue 4, pp. 715-20, (1989) (PubMed).

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: E-Selectin, CD62-E (SELE Products)

Synonyms: SELE antibody, CD62E antibody, ELAM antibody, ELAM1 antibody, ESEL antibody, LECAM2 antibody, E-selectin antibody, Elam antibody, selectin E antibody, selectin, endothelial cell antibody, SELE antibody, Sele antibody

Background: ENA1 reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). The antibody reacts with human endothelial cells activated with TNF-alpha, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the E-selectin gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.

Pathways: Thromboxane A2 Receptor Signaling