C6 antibody

Catalog No. ABIN2192122

Product Details anti-C6 Antibody

Target: C6 (Complement Component 6 (C6))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This C6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: 3G11

Application Details

Application Notes: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-C6 antibody (ABIN2192122)

Sato, Van Dixhoorn, Prins, Mooney, Verhagen, Muizert, Savill, Van Es, Daha: "The terminal sequence of complement plays an essential role in antibody-mediated renal cell apoptosis." in: Journal of the American Society of Nephrology : JASN, Vol. 10, Issue 6, pp. 1242-52, (1999) (PubMed).

Baker, Ochi, Schulze, Johnson, Campbell, Couser: "Depletion of C6 prevents development of proteinuria in experimental membranous nephropathy in rats." in: The American journal of pathology, Vol. 135, Issue 1, pp. 185-94, (1989) (PubMed).

Schulze, Baker, Perkinson, Johnson, Ochi, Stahl, Couser: "Increased urinary excretion of C5b-9 distinguishes passive Heymann nephritis in the rat." in: Kidney international, Vol. 35, Issue 1, pp. 60-8, (1989) (PubMed).

Target Details for C6

Target: C6 (Complement Component 6 (C6))

Abstract: C6 Products

Synonyms: zgc:64073 antibody, MGC82168 antibody, c6 antibody, MGC89471 antibody, C6 antibody, AW111623 antibody, complement component 6 antibody, complement C6 antibody, complement component 6, gene 1 L homeolog antibody, complement component 6, gene 2 antibody, c6 antibody, C6 antibody, c6.1.L antibody, c6.2 antibody

Background: The monoclonal antibody 3G11 reacts specifically with rat C6. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 membrane attack complex. Complement component C6 is part of this C5b-9 complex. This complex is assembled from five precursor molecules in the serum. The final step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. The rat remnant kidney model had been widely used to identify mechanisms responsible for the progression of renal disease. Proteinuria is nephrotoxic and contributes to the progression of renal injury. Activation of complement in proteinuric urine results in tubular and interstitial damage. In C6- deficient rats, acute interstitial changes resolved despite ongoing proteinuria. This suggests that C6 mediates chronic progression of tubulointerstitial damage in rats with remnant kidneys. The monoclonal antibody 3G11 was raised by immunization of mice with C6 isolated from rat serum. Monoclonal antibody 3G11 can be used as detector in immuno assays detect C5b-9 in both serum and urine.