Endothelial Cells antibody

Catalog No. ABIN2192103

Product Details anti-Endothelial Cells Antibody

Target: Endothelial Cells

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endothelial Cells antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Cross-Reactivity (Details): Cross reactivity: Goat : No, Human : No, Mouse : No, Rabbit : No, Sheep : No

Sterility: 0.2 μm filtered

Clone: RECA-1

Isotype: IgG1

Application Details

Application Notes: For immunohistology and flow cytometry dilutions to be used depends on tissue type and on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Glomerular endothelial cells, RHEC cell line control Negative Vascular smooth muscle cells, fibroblasts control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-Endothelial Cells antibody (ABIN2192103)

Louboutin, Reyes, Agrawal, Maxwell, Van Bockstaele, Strayer: "Blood-brain barrier abnormalities caused by exposure to HIV-1 gp120--protection by gene delivery of antioxidant enzymes." in: Neurobiology of disease, Vol. 38, Issue 2, pp. 313-25, (2010) (PubMed).

Pradhan, Umezu, Fukagawa: "Heme-oxygenase upregulation ameliorates angiotensin II-induced tubulointerstitial injury and salt-sensitive hypertension." in: American journal of nephrology, Vol. 26, Issue 6, pp. 552-61, (2007) (PubMed).

Johnson, Gordon, Suga, Duijvestijn, Griffin, Bidani: "Renal injury and salt-sensitive hypertension after exposure to catecholamines." in: Hypertension, Vol. 34, Issue 1, pp. 151-9, (1999) (PubMed).

Derhaag, Duijvestijn, Van Breda Vriesman: "Heart EC respond heterogeneous on cytokine stimulation in ICAM-1 and VCAM-1, but not in MHC expression. A study with 3 rat heart endothelial cell (RHEC) lines." in: Endothelium : journal of endothelial cell research, Vol. 5, Issue 4, pp. 307-19, (1998) (PubMed).

Duijvestijn, van Goor, Klatter, Majoor, van Bussel, van Breda Vriesman: "Antibodies defining rat endothelial cells: RECA-1, a pan-endothelial cell-specific monoclonal antibody." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 66, Issue 4, pp. 459-66, (1992) (PubMed).

Target Details for Endothelial Cells

Target: Endothelial Cells

Abstract: Endothelial Cells Products

Background: The monoclonal antibody RECA-1 reacts with Rat Endothelial Cell Antigen (RECA), a cell surface antigen (MCA970R) on rat endothelial cells. Endothelial cells (EC) line the interior of all blood vessels and are the key players in the angiogenesis cascade. EC are the first cells and barrier that vehicles or medicines encounter after systemic delivery. Furthermore, they have a signaling function to the cells of the immune system to indicate the status of the surrounding tissue. RECA-1is at least reactive with 1 n u the rat MHC-haplotype, Lewis (TR-1 ), BN (RT-1 ) and OA (RT-1 ). RECA-1 antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of the RECA-1 monoclonal antibody was seen with other cell types e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.