AOC3 antibody

Catalog No. ABIN2192061

Product Details anti-AOC3 Antibody

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AOC3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Cross-Reactivity (Details): Cross reactivity: Rat : Yes

Sterility: 0.2 μm filtered

Clone: 174-5

Isotype: IgG1

Application Details

Application Notes: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system chemistry, , applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Ax cells stably transfected with VAP-1 cDNA. (Ref. 1) VAP control Negative Mock transfected Ax cells. (Ref. 1) control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-AOC3 antibody (ABIN2192061)

Autio, Ujula, Luoto, Salomäki, Jalkanen, Roivainen: "PET imaging of inflammation and adenocarcinoma xenografts using vascular adhesion protein 1 targeting peptide 68Ga-DOTAVAP-P1: comparison with 18F-FDG." in: European journal of nuclear medicine and molecular imaging, Vol. 37, Issue 10, pp. 1918-25, (2010) (PubMed).

Martelius, Salaspuro, Salmi, Krogerus, Höckerstedt, Jalkanen, Lautenschlager: "Blockade of vascular adhesion protein-1 inhibits lymphocyte infiltration in rat liver allograft rejection." in: The American journal of pathology, Vol. 165, Issue 6, pp. 1993-2001, (2004) (PubMed).

Target Details for AOC3

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Alternative Name: Vascular Adhesion Protein-1 (AOC3 Products)

Synonyms: HPAO antibody, SSAO antibody, VAP-1 antibody, VAP1 antibody, aoc2 antibody, BOLAO antibody, amine oxidase, copper containing 3 antibody, amine oxidase, copper containing 3 L homeolog antibody, AOC3 antibody, aoc3.L antibody, Aoc3 antibody

Background: The monoclonal antibody 174-5 recognises human Vascular Adhesion Protein-1 (VAP-1), which is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end- products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity. Elevated sVAP- 1serum levels have been described in several inflammatory diseases as well as colorectal cancer. Moreover, diminished insulin secretion appears to increase the concentration of soluble VAP-1 in plasma. Therefore, VAP-1 might be an interesting diagnostic marker as well therapeutic target for modulating inflammation.The monoclonal antibody 174-5 has been shown to cross-react with rat VAP- 1 and to inhibit lymphocyte infiltration in rat liver allograft rejection. Aliases membrane primary amine oxidase, AOC3, SSAO Immunogen Purified vessels from human peripheral lymph nodes (ref 1)

Pathways: Feeding Behaviour