MARCO antibody

Catalog No. ABIN2192057

Product Details anti-MARCO Antibody

Target: MARCO (Macrophage Receptor with Collagenous Structure (MARCO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MARCO antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Cross-Reactivity (Details): Cross reactivity: Bovine : Yes, Chicken : No

Sterility: 0.2 μm filtered

Clone: PLK-1

Isotype: IgG3

Application Details

Application Notes: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Human alveolar macrophages control Negative All other human cells control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-MARCO antibody (ABIN2192057)

Arredouani, Palecanda, Koziel, Huang, Imrich, Sulahian, Ning, Yang, Pikkarainen, Sankala, Vargas, Takeya, Tryggvason, Kobzik: "MARCO is the major binding receptor for unopsonized particles and bacteria on human alveolar macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6058-64, (2005) (PubMed).

Bunn, Kobzik, Grigg: "MARCO expression on pediatric alveolar macrophages." in: Cytometry. Part B, Clinical cytometry, Vol. 60, Issue 1, pp. 54-6, (2004) (PubMed).

Palecanda, Kobzik: "Receptors for unopsonized particles: the role of alveolar macrophage scavenger receptors." in: Current molecular medicine, Vol. 1, Issue 5, pp. 589-95, (2002) (PubMed).

Target Details for MARCO

Target: MARCO (Macrophage Receptor with Collagenous Structure (MARCO))

Alternative Name: Marco (MARCO Products)

Synonyms: AI323439 antibody, Ly112 antibody, Scara2 antibody, SCARA2 antibody, macrophage receptor with collagenous structure antibody, MARCO antibody, Marco antibody

Background: The monoclonal antibody PLK-1 recognizes the macrophage receptor with collagenous structure (MARCO). The scavenger receptors (SRs) expressed by macrophages are thought to play an important role in the immune response against bacteria by mediating ligand binding and phagocytosis. SRs can be divided into three different classes based upon their structural properties, which are termed SR-A, SR-B and SR-C. SRs-A are homotrimeric glycoproteins composed of 77 kDa monomers subdivided into 3 types.. The molecular structure of MARCO resembles that of SR-A type I, containing a triple-helical collagenous domain and a scavenger receptor cysteine-rich (SRCR) domain at the C terminus. MARCO is only expressed in some subpopulations of macrophages, although it's expression can be strongly upregulated during infection or LPS treatment. Furthermore, MARCO is, like sinusoidal endothelial cell markers DC-SIGNR, LYVE-1 and stabilin-2, expressed by sinusoidal endothelial cells in lymph node. MARCO expressed by alveolar macrophages seems to play an important role in response to inhaled particles and airborne pathogens. The monoclonal antibody PLK-1 binds specifically to MARCO, and has been shown to partially block ligand binding. Aliases SCARA2, scavenger receptor class A member 2, macrophage receptor with collagenous structure Immunogen Human alveolar macrophages (recognizes domain V between residues 420 and 431)

Pathways: Activation of Innate immune Response