Desmoglein 2 antibody

Catalog No. ABIN2191994

Product Details anti-Desmoglein 2 Antibody

Target: Desmoglein 2 (DSG2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity (Details): Cross reactivity: Human desmoglein-1 : No, Human desmoglein-3 : No

Sterility: 0.2 μm filtered

Clone: 6D8

Isotype: IgG1

Application Details

Application Notes: For immune fluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Positive A431 Cells control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-Desmoglein 2 antibody (ABIN2191994)

Ota, Amagai, Watanabe, Nishikawa: "No involvement of IgG autoantibodies against extracellular domains of desmoglein 2 in paraneoplastic pemphigus or inflammatory bowel diseases." in: Journal of dermatological science, Vol. 32, Issue 2, pp. 137-41, (2003) (PubMed).

Wahl, Sacco, McGranahan-Sadler, Sauppé, Wheelock, Johnson: "Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains." in: Journal of cell science, Vol. 109 ( Pt 5), pp. 1143-54, (1996) (PubMed).

Target Details for Desmoglein 2

Target: Desmoglein 2 (DSG2)

Alternative Name: Desmoglein-2 (DSG2 Products)

Synonyms: DSG2 antibody, dsc antibody, dsga antibody, si:dkeyp-51f12.6 antibody, wu:fj23f02 antibody, ARVC10 antibody, ARVD10 antibody, CDHF5 antibody, CMD1BB antibody, HDGC antibody, AA408168 antibody, D18Ertd293e antibody, desmoglein 2 antibody, desmoglein 2, tandem duplicate 1 antibody, DSG2 antibody, dsg2.1 antibody, dsg2 antibody, Dsg2 antibody

Background: The monoclonal antibody 6D8 recognizes the extracellular domain of human desmoglein-2. Desmogleins and desmocollins are members of the cadherin family of transmembrane proteins that together make up the core of the desmosome, a structure that provides transmembrane strength to tissues undergoing mechanical stress. The desmosomal cadherins, desmogleins and desmocollins, mediate calcium-dependent cell-cell adhesion by forming homotypic and heterotypic interactions with one another. The multiprotein desmosomal complex also includes the cytoplasmic desmosomal plaque proteins plakoglobin, phakophilins, and desmoplakin, which bind to the intracellular domain of the desmogleins and function to anchor the keratin intermediate filament network to site of cell-cell contacts. In human, four desmogleins have been identified (Dsg1-4). Desmogleins are synthesized with a signal peptide that directs them to the endoplasmic reticulum and a proregion that is removed during protein processing. The mature protein includes four highly conserved extracellular domains (EC 1-4) and a fifth membrane proximal, more variable EC domain that is referred to as the "extracellular anchor domain. Desmoglein-2 is expressed on various cells including simple epithelia and myocardium, tumors and and many cell cultures. Desmogleins play critical roles in cell adhesion and skin blistering diseases, as they are the target antigens of autoimmune antibodies and bacterial toxins. Desmosomal dysfunction has been implicated in a number of diseases, including striate palmoplantar keratoderma, skin fragility, and ectodermal dysplasia, and most recently arrhythmic right ventricular cardiomyopathy (ARVC). Aliases Dsg2, Cadherin family member 5, HDGC Immunogen Extracellular EC4 domain

Pathways: Cell-Cell Junction Organization