Polymyxin B antibody

Catalog No. ABIN2191915

Product Details anti-Polymyxin B Antibody

Target: Polymyxin B

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: This Polymyxin B antibody is un-conjugated

Application: Western Blotting (WB)

Endotoxin Level: Low endotoxin level

Clone: 45

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Polymyxin B antibody (ABIN2191915)

Appelmelk, Su, Verweij-van Vught, Thijs, MacLaren: "Polymyxin B-horseradish peroxidase conjugates as tools in endotoxin research." in: Analytical biochemistry, Vol. 207, Issue 2, pp. 311-6, (1993) (PubMed).

Target Details for Polymyxin B

Target: Polymyxin B

Abstract: Polymyxin B Products

Target Type: Chemical

Background: The monoclonal antibody 45 reacts with Polymyxin B. The antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody 45 enables the possibilities to study quantitatively the interaction of PMB and LPS.