Lipid A antibody

Catalog No. ABIN2191914

Product Details

Target: Lipid A

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Endotoxin Level: Low endotoxin level

Clone: 43

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Chou, Chuang, Eng, Chen, Wang, Chen, Lin: "Endotoxin and CD14 in the progression of biliary atresia." in: Journal of translational medicine, Vol. 8, pp. 138, (2011) (PubMed).

Kuhn, Brade, Appelmelk, Kusumoto, Rietschel, Brade: "The antibody reactivity of monoclonal lipid A antibodies is influenced by the acylation pattern of lipid A and the assay system employed." in: Immunobiology, Vol. 189, Issue 5, pp. 457-71, (1994) (PubMed).

Kuhn, Brade, Appelmelk, Kusumoto, Rietschel, Brade: "Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A." in: Infection and immunity, Vol. 60, Issue 6, pp. 2201-10, (1992) (PubMed).

Target Details

Target: Lipid A

Target Type: Chemical

Background: The monoclonal antibody 43 reacts with the hydrophilic backbone of free lipid A. This antibody only reacts with lipid A not covalent bound to LPS. LPS is chemically composed of a lipid part, lipid A, which is responsible for the endotoxic activities of LPS, and a heteropolysaccharide which generally consists of an oligosaccharide, termed the core region, and, in some bacterial families, of the so-called O chain, composed of repeating oligosaccharide units.