CD51/CD61 antibody (FITC)

Catalog No. ABIN2191903

Product Details anti-CD51/CD61 Antibody (FITC)

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51/CD61 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity: Human : Yes

Clone: BV3

Isotype: IgG1

Application Details

Application Notes: For flow cytometry and innumihistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive HUVEC cells control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 1 % bovine serum albumin and 0.02 % sodium azide 2* 3 1

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-CD51/CD61 antibody (FITC) (ABIN2191903)

Merkel, Germershaus, Wada, Tarcha, Merdan, Kissel: "Integrin alphaVbeta3 targeted gene delivery using RGD peptidomimetic conjugates with copolymers of PEGylated poly(ethylene imine)." in: Bioconjugate chemistry, Vol. 20, Issue 6, pp. 1270-80, (2009) (PubMed).

Dare, Griffith, Poitras, Wang, Dervin, Giulivi, Hincke: "Fibrin sealants from fresh or fresh/frozen plasma as scaffolds for in vitro articular cartilage regeneration." in: Tissue engineering. Part A, Vol. 15, Issue 8, pp. 2285-97, (2009) (PubMed).

Newton, Girgenti, Collier, Duman: "Electroconvulsive seizure increases adult hippocampal angiogenesis in rats." in: The European journal of neuroscience, Vol. 24, Issue 3, pp. 819-28, (2006) (PubMed).

Target Details for CD51/CD61

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Abstract: ITGAV/ITGB3 Products

Synonyms: CD51 antibody, MSK8 antibody, VNRA antibody, VTNR antibody, integrin subunit alpha V antibody, ITGAV antibody

Background: The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells. Integrins are a superfamily of αβ heterodimeric cell-surface adhesion receptors found in many species. They are expressed on a variety of cells and mediate numerous physiological processes, including inflammation, migration, adhesion and proliferation. The β3 family consist of 2 members: αIIbβ3 and αvβ3, which mediate cell-cell and cell-ECM interactions and are important for cellular migration, regulation of gene expression, cell survival, adhesion and differentiation. All processes which are involved in tissue development, angiogenesis and thrombosis. Each subunit consist of an extracellular domain, a single transmembrane segment and a cytoplasmic tail. They connect to the actin cytoskeleton via adaptor proteins that bind theircytoplasmic tails. Cell matrix adhesions also act as signaling units by their capacity to organize the actin cytoskeleton and to accumulate various signaling intermediates. Integrin αvβ3 was originally identified as the vitronectin receptor. Nevertheless, other ligands include fibrinogen, fibronectin, laminin, thrombospondin, Von Willebrand factor, tenascin, osteopontin and several forms of collagen. The interactions of integrin αvβ3 to those ligands is mediated by the RGD (Arg-Gly-Asp) sequence motif present in these proteins. Deregulation of β3 integrins is involved in e.g. autoimmune diseases, cardiovascular disorders, transplant rejection and tumorigenesis. In contribution to the latter, integrin αvβ3 contribute by supporting growth of small (tumor) blood vessels thereby potentiating the metastatic potential. Overexpression of integrin αvβ3 has been demonstrated in various tumors and activated endothelium. Aliases Vitronectin receptor, integrin αvβ3, cd51/cd61