TREM2
Reactivity: Mouse, Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date
12 months
Koga, Inui, Inoue, Kim, Suematsu, Kobayashi, Iwata, Ohnishi, Matozaki, Kodama, Taniguchi, Takayanagi, Takai: "Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis." in: Nature, Vol. 428, Issue 6984, pp. 758-63, (2004) (PubMed).
Target
TREM2
(Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))
Alternative Name
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2 Products)
The monoclonal antibody 6E9 recognizes mouse membrane-bound as well as soluble triggering receptor expressed on myeloid cells-2 (TREM-2). TREM-2 is a 26 kDa transmembrane glycoprotein that consists of a single extracellular immunoglobulin-like domain, a transmembrane region with a charged lysine residue and a short cytoplasmic tail. It associates with DNAX-activation protein 12 (DAP12) for signaling and function. TREM-2 is expressed on immature monocyte-derived dendritic cells. After activation by microbial products or tumor necrosis factor (TNF) and TNF-related proteins, dendritic cells downregulate the expression of TREM-2. TREM-2 is also expressed by osteoclasts and microglia, where it is involved in bone modeling and brain function, respectively. Another role of TREM-2 might be promoting the removal of apoptotic cells, organic matrix and macromolecules by microglia. Defects in TREM-2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). TREM-2, like TREM-1, can be cleaved on the membrane to release a soluble form of TREM-2 (sTREM-2). Elevated levels of sTREM-2 in CSF of multiple sclerosis patients have been detected. This elevated level may inhibit the anti-inflammatory function of the membrane-bound receptor suggesting sTREM-2 to be a possible target for future therapies. Aliases Triggering receptor expressed on monocytes 2, TREM2 Immunogen Mouse recombinant TREM-2 protein