Red Blood Cell antibody

Catalog No. ABIN2191860

Product Details anti-Red Blood Cell Antibody

Target: Red Blood Cell (RBC)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Red Blood Cell antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Cross-Reactivity (Details): Cross reactivity: Rat, sheep, rabit, chicken, human : No

Sterility: 0.2 μm filtered

Clone: 34-3C

Isotype: IgG2a

Application Details

Application Notes: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Mouse erythrocytes control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-Red Blood Cell antibody (ABIN2191860)

Baudino, Nimmerjahn, Azeredo da Silveira, Martinez-Soria, Saito, Carroll, Ravetch, Verbeek, Izui: "Differential contribution of three activating IgG Fc receptors (FcgammaRI, FcgammaRIII, and FcgammaRIV) to IgG2a- and IgG2b-induced autoimmune hemolytic anemia in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 3, pp. 1948-53, (2008) (PubMed).

Fossati-Jimack, Azeredo da Silveira, Moll, Kina, Kuypers, Oldenborg, Reininger, Izui: "Selective increase of autoimmune epitope expression on aged erythrocytes in mice: implications in anti-erythrocyte autoimmune responses." in: Journal of autoimmunity, Vol. 18, Issue 1, pp. 17-25, (2002) (PubMed).

Azeredo da Silveira, Kikuchi, Fossati-Jimack, Moll, Saito, Verbeek, Botto, Walport, Carroll, Izui: "Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high affinity anti-erythrocyte autoantibody." in: The Journal of experimental medicine, Vol. 195, Issue 6, pp. 665-72, (2002) (PubMed).

Shibata, Berney, Reininger, Chicheportiche, Ozaki, Shirai, Izui: "Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune hemolytic anemia by two distinct pathogenic mechanisms." in: International immunology, Vol. 2, Issue 12, pp. 1133-41, (1991) (PubMed).

Target Details for Red Blood Cell

Target: Red Blood Cell (RBC)

Alternative Name: Rbc (RBC Products)

Background: The monoclonal antibody 34-3C recognizes an exposed surface determinant of intact red blood cells (RBC). The high-affinity anti-RBC monoclonal antibody efficiently bind to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity. The monoclonal antibody only recognises antigenic determinants expressed on Mouse RBC and not on other species of RBC. Immunogen RBC